Articles | Volume 2, issue 1
https://doi.org/10.5194/mr-2-1-2021
https://doi.org/10.5194/mr-2-1-2021
Research article
 | 
06 Jan 2021
Research article |  | 06 Jan 2021

Phosphoserine for the generation of lanthanide-binding sites on proteins for paramagnetic nuclear magnetic resonance spectroscopy

Sreelakshmi Mekkattu Tharayil, Mithun Chamikara Mahawaththa, Choy-Theng Loh, Ibidolapo Adekoya, and Gottfried Otting

Viewed

Total article views: 3,386 (including HTML, PDF, and XML)
HTML PDF XML Total Supplement BibTeX EndNote
2,187 1,019 180 3,386 609 174 231
  • HTML: 2,187
  • PDF: 1,019
  • XML: 180
  • Total: 3,386
  • Supplement: 609
  • BibTeX: 174
  • EndNote: 231
Views and downloads (calculated since 04 Nov 2020)
Cumulative views and downloads (calculated since 04 Nov 2020)

Viewed (geographical distribution)

Total article views: 3,386 (including HTML, PDF, and XML) Thereof 3,155 with geography defined and 231 with unknown origin.
Country # Views %
  • 1
1
 
 
 
 

Cited

Saved (final revised paper)

Latest update: 04 May 2026
Download
Short summary
A new way is presented for creating lanthanide binding sites on proteins using site-specifically introduced phosphoserine residues. The paramagnetic effects of lanthanides generate long-range effects, which contain structural information and are readily measured by NMR spectroscopy. Excellent correlations between experimentally observed and back-calculated pseudocontact shifts attest to very good immobilization of the lanthanide ions relative to the proteins.
Share