Phosphoserine for the generation of lanthanide-binding sites on proteins for paramagnetic nuclear magnetic resonance spectroscopy
Sreelakshmi Mekkattu Tharayil,Mithun Chamikara Mahawaththa,Choy-Theng Loh,Ibidolapo Adekoya,and Gottfried Otting
Sreelakshmi Mekkattu Tharayil
ARC Centre of Excellence for Innovations in Peptide and Protein
Science, Research School of Chemistry, Australian National University,
Canberra ACT 2601, Australia
Mithun Chamikara Mahawaththa
ARC Centre of Excellence for Innovations in Peptide and Protein
Science, Research School of Chemistry, Australian National University,
Canberra ACT 2601, Australia
Choy-Theng Loh
ARC Centre of Excellence for Innovations in Peptide and Protein
Science, Research School of Chemistry, Australian National University,
Canberra ACT 2601, Australia
ARC Centre of Excellence for Innovations in Peptide and Protein
Science, Research School of Chemistry, Australian National University,
Canberra ACT 2601, Australia
ARC Centre of Excellence for Innovations in Peptide and Protein
Science, Research School of Chemistry, Australian National University,
Canberra ACT 2601, Australia
Viewed
Total article views: 1,562 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
1,026
492
44
1,562
312
34
37
HTML: 1,026
PDF: 492
XML: 44
Total: 1,562
Supplement: 312
BibTeX: 34
EndNote: 37
Views and downloads (calculated since 04 Nov 2020)
Cumulative views and downloads
(calculated since 04 Nov 2020)
Total article views: 1,195 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
795
360
40
1,195
201
33
35
HTML: 795
PDF: 360
XML: 40
Total: 1,195
Supplement: 201
BibTeX: 33
EndNote: 35
Views and downloads (calculated since 06 Jan 2021)
Cumulative views and downloads
(calculated since 06 Jan 2021)
Total article views: 367 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
231
132
4
367
111
1
2
HTML: 231
PDF: 132
XML: 4
Total: 367
Supplement: 111
BibTeX: 1
EndNote: 2
Views and downloads (calculated since 04 Nov 2020)
Cumulative views and downloads
(calculated since 04 Nov 2020)
Viewed (geographical distribution)
Total article views: 1,562 (including HTML, PDF, and XML)
Thereof 1,430 with geography defined
and 132 with unknown origin.
Total article views: 1,195 (including HTML, PDF, and XML)
Thereof 1,124 with geography defined
and 71 with unknown origin.
Total article views: 367 (including HTML, PDF, and XML)
Thereof 306 with geography defined
and 61 with unknown origin.
A new way is presented for creating lanthanide binding sites on proteins using site-specifically introduced phosphoserine residues. The paramagnetic effects of lanthanides generate long-range effects, which contain structural information and are readily measured by NMR spectroscopy. Excellent correlations between experimentally observed and back-calculated pseudocontact shifts attest to very good immobilization of the lanthanide ions relative to the proteins.
A new way is presented for creating lanthanide binding sites on proteins using site-specifically...