Articles | Volume 2, issue 1
Magn. Reson., 2, 1–13, 2021
https://doi.org/10.5194/mr-2-1-2021

Special issue: Robert Kaptein Festschrift

Magn. Reson., 2, 1–13, 2021
https://doi.org/10.5194/mr-2-1-2021

Research article 06 Jan 2021

Research article | 06 Jan 2021

Phosphoserine for the generation of lanthanide-binding sites on proteins for paramagnetic nuclear magnetic resonance spectroscopy

Sreelakshmi Mekkattu Tharayil et al.

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Short summary
A new way is presented for creating lanthanide binding sites on proteins using site-specifically introduced phosphoserine residues. The paramagnetic effects of lanthanides generate long-range effects, which contain structural information and are readily measured by NMR spectroscopy. Excellent correlations between experimentally observed and back-calculated pseudocontact shifts attest to very good immobilization of the lanthanide ions relative to the proteins.