Phosphoserine for the generation of lanthanide-binding sites on proteins for paramagnetic nuclear magnetic resonance spectroscopy
Sreelakshmi Mekkattu Tharayil,Mithun Chamikara Mahawaththa,Choy-Theng Loh,Ibidolapo Adekoya,and Gottfried Otting
Sreelakshmi Mekkattu Tharayil
ARC Centre of Excellence for Innovations in Peptide and Protein
Science, Research School of Chemistry, Australian National University,
Canberra ACT 2601, Australia
Mithun Chamikara Mahawaththa
ARC Centre of Excellence for Innovations in Peptide and Protein
Science, Research School of Chemistry, Australian National University,
Canberra ACT 2601, Australia
Choy-Theng Loh
ARC Centre of Excellence for Innovations in Peptide and Protein
Science, Research School of Chemistry, Australian National University,
Canberra ACT 2601, Australia
ARC Centre of Excellence for Innovations in Peptide and Protein
Science, Research School of Chemistry, Australian National University,
Canberra ACT 2601, Australia
ARC Centre of Excellence for Innovations in Peptide and Protein
Science, Research School of Chemistry, Australian National University,
Canberra ACT 2601, Australia
Viewed
Total article views: 3,041 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
1,991
893
157
3,041
565
157
197
HTML: 1,991
PDF: 893
XML: 157
Total: 3,041
Supplement: 565
BibTeX: 157
EndNote: 197
Views and downloads (calculated since 04 Nov 2020)
Cumulative views and downloads
(calculated since 04 Nov 2020)
Total article views: 2,578 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
1,723
705
150
2,578
383
152
188
HTML: 1,723
PDF: 705
XML: 150
Total: 2,578
Supplement: 383
BibTeX: 152
EndNote: 188
Views and downloads (calculated since 06 Jan 2021)
Cumulative views and downloads
(calculated since 06 Jan 2021)
Total article views: 463 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
268
188
7
463
182
5
9
HTML: 268
PDF: 188
XML: 7
Total: 463
Supplement: 182
BibTeX: 5
EndNote: 9
Views and downloads (calculated since 04 Nov 2020)
Cumulative views and downloads
(calculated since 04 Nov 2020)
Viewed (geographical distribution)
Total article views: 3,041 (including HTML, PDF, and XML)
Thereof 2,819 with geography defined
and 222 with unknown origin.
Total article views: 2,578 (including HTML, PDF, and XML)
Thereof 2,419 with geography defined
and 159 with unknown origin.
Total article views: 463 (including HTML, PDF, and XML)
Thereof 400 with geography defined
and 63 with unknown origin.
A new way is presented for creating lanthanide binding sites on proteins using site-specifically introduced phosphoserine residues. The paramagnetic effects of lanthanides generate long-range effects, which contain structural information and are readily measured by NMR spectroscopy. Excellent correlations between experimentally observed and back-calculated pseudocontact shifts attest to very good immobilization of the lanthanide ions relative to the proteins.
A new way is presented for creating lanthanide binding sites on proteins using site-specifically...
