Articles | Volume 2, issue 1
Magn. Reson., 2, 1–13, 2021

Special issue: Robert Kaptein Festschrift

Magn. Reson., 2, 1–13, 2021

Research article 06 Jan 2021

Research article | 06 Jan 2021

Phosphoserine for the generation of lanthanide-binding sites on proteins for paramagnetic nuclear magnetic resonance spectroscopy

Sreelakshmi Mekkattu Tharayil et al.

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Cited articles

Abdelkader, E. H., Yao, X., Feintuch, A., Adams, L. A., Aurelio, L., Graham, B., Goldfarb, D., and Otting, G.: Pulse EPR-enabled interpretation of scarce pseudocontact shifts induced by lanthanide binding tags, J. Biomol. NMR, 64, 39–51,, 2016. 
Baneyx, F. and Mujacic, M.: Recombinant protein folding and misfolding in Escherichia coli, Nat. Biotechnol., 22, 1399–1408,, 2004. 
Barthelmes, D., Gränz, M., Barthelmes, K., Allen, K. N., Imperiali, B., Prisner, T., and Schwalbe, H.: Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy, J. Biomol. NMR, 63, 275–282,, 2015. 
Barthelmes, D., Barthelmes, K., Schnorr, K., Jonker, H. R. A., Bodmer, B., Allen, K. N., Imperiali, B., and Schwalbe, H.: Conformational dynamics and alignment properties of loop lanthanide-binding-tags (LBTs) studied in interleukin-1β, J. Biomol. NMR, 68, 187–194,, 2017. 
Barthelmes, K., Reynolds, A. M., Peisach, E., Jonker, H. R. A., DeNunzio, N. J., Allen, K. N., Imperiali, B., and Schwalbe, H.: Engineering encodable lanthanide-binding tags into loop regions of proteins, J. Am. Chem. Soc., 133, 808–819,, 2011. 
Short summary
A new way is presented for creating lanthanide binding sites on proteins using site-specifically introduced phosphoserine residues. The paramagnetic effects of lanthanides generate long-range effects, which contain structural information and are readily measured by NMR spectroscopy. Excellent correlations between experimentally observed and back-calculated pseudocontact shifts attest to very good immobilization of the lanthanide ions relative to the proteins.