Phosphoserine for the generation of lanthanide-binding sites on proteins for paramagnetic nuclear magnetic resonance spectroscopy

Mekkattu Tharayil, Sreelakshmi; Mahawaththa, Mithun Chamikara; Loh, Choy-Theng; Adekoya, Ibidolapo; Otting, Gottfried

doi:https://doi.org/10.5194/mr-2-1-2021

Articles | Volume 2, issue 1

https://doi.org/10.5194/mr-2-1-2021

© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.

Special issue:

Robert Kaptein Festschrift

https://doi.org/10.5194/mr-2-1-2021

© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 2, issue 1

Research article

|

06 Jan 2021

Research article |

| 06 Jan 2021

Phosphoserine for the generation of lanthanide-binding sites on proteins for paramagnetic nuclear magnetic resonance spectroscopy

Sreelakshmi Mekkattu Tharayil, Mithun Chamikara Mahawaththa, Choy-Theng Loh, Ibidolapo Adekoya, and Gottfried Otting

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Final revised paper (published on 06 Jan 2021)
Supplement to the final revised paper
Preprint (discussion started on 04 Nov 2020)
Supplement to the preprint

Interactive discussion

Status: closed

AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment

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- Supplement

RC1: 'Referee comments', Marcellus Ubbink, 17 Nov 2020
- AC1: 'Response to the comments made by Marcellus Ubbink', Gottfried Otting, 30 Nov 2020
  - AC3: 'revised Figure S1, reporting details of ITC data', Gottfried Otting, 30 Nov 2020
RC2: 'RC on "Phosphoserine for the generation of lanthanide binding sites on proteins for paramagnetic NMR"', Claudio Luchinat, 25 Nov 2020
- AC2: 'Response to the comments made by Claudio Luchinat', Gottfried Otting, 30 Nov 2020
  - RC4: 'Reply to the response', Claudio Luchinat, 30 Nov 2020
RC3: 'Reply to author comments', Marcellus Ubbink, 30 Nov 2020

Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision

AR by Gottfried Otting on behalf of the Authors (04 Dec 2020) Author's response Manuscript

ED: Publish subject to minor revisions (review by editor) (09 Dec 2020) by Rolf Boelens

AR by Gottfried Otting on behalf of the Authors (11 Dec 2020) Author's response Author's tracked changes Manuscript

ED: Publish as is (12 Dec 2020) by Rolf Boelens

AR by Gottfried Otting on behalf of the Authors (13 Dec 2020)

Post-review adjustments

AA: Author's adjustment | EA: Editor approval

AA by Gottfried Otting on behalf of the Authors (23 Dec 2020) Author's adjustment Manuscript

EA: Adjustments approved (23 Dec 2020) by Rolf Boelens

Short summary

A new way is presented for creating lanthanide binding sites on proteins using site-specifically introduced phosphoserine residues. The paramagnetic effects of lanthanides generate long-range effects, which contain structural information and are readily measured by NMR spectroscopy. Excellent correlations between experimentally observed and back-calculated pseudocontact shifts attest to very good immobilization of the lanthanide ions relative to the proteins.