Articles | Volume 2, issue 1
Magn. Reson., 2, 1–13, 2021
https://doi.org/10.5194/mr-2-1-2021

Special issue: Robert Kaptein Festschrift

Magn. Reson., 2, 1–13, 2021
https://doi.org/10.5194/mr-2-1-2021

Research article 06 Jan 2021

Research article | 06 Jan 2021

Phosphoserine for the generation of lanthanide-binding sites on proteins for paramagnetic nuclear magnetic resonance spectroscopy

Sreelakshmi Mekkattu Tharayil et al.

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Interactive discussion

Status: closed
Status: closed
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
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Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision
AR by Gottfried Otting on behalf of the Authors (04 Dec 2020)  Author's response    Manuscript
ED: Publish subject to minor revisions (review by editor) (09 Dec 2020) by Rolf Boelens
AR by Gottfried Otting on behalf of the Authors (11 Dec 2020)  Author's response    Author's tracked changes    Manuscript
ED: Publish as is (12 Dec 2020) by Rolf Boelens

Post-review adjustments

AA: Author's adjustment | EA: Editor approval
AA by Gottfried Otting on behalf of the Authors (23 Dec 2020)   Author's adjustment   Manuscript
EA: Adjustments approved (23 Dec 2020) by Rolf Boelens
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Short summary
A new way is presented for creating lanthanide binding sites on proteins using site-specifically introduced phosphoserine residues. The paramagnetic effects of lanthanides generate long-range effects, which contain structural information and are readily measured by NMR spectroscopy. Excellent correlations between experimentally observed and back-calculated pseudocontact shifts attest to very good immobilization of the lanthanide ions relative to the proteins.