Articles | Volume 2, issue 2
https://doi.org/10.5194/mr-2-571-2021
https://doi.org/10.5194/mr-2-571-2021
Research article
 | 
12 Jul 2021
Research article |  | 12 Jul 2021

Rapid measurement of heteronuclear transverse relaxation rates using non-uniformly sampled R1ρ accordion experiments

Sven Wernersson, Göran Carlström, Andreas Jakobsson, and Mikael Akke

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Field: Liquid-state NMR | Topic: Pulse-sequence development
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Cited articles

Ahlner, A., Carlsson, M., Jonsson, B. H., and Lundström, P.: PINT: A software for integration of peak volumes and extraction of relaxation rates, J. Biomol. NMR, 56, 191–202, https://doi.org/10.1007/s10858-013-9737-7, 2013. 
Akke, M. and Palmer, A. G.: Monitoring Macromolecular Motions on Microsecond–Millisecond Time Scales by R1r–R1 Constant-Relaxation-Time NMR Spectroscopy, J. Am. Chem. Soc., 118, 911–912, 1996. 
Alderson, T. R. and Kay, L. E.: Unveiling invisible protein states with NMR spectroscopy, Curr. Opin. Struct. Biol., 60, 39–49, https://doi.org/10.1016/j.sbi.2019.10.008, 2020. 
Bodenhausen, G. and Ernst, R. R.: The accordion experiment, a simple approach to three-dimensional NMR spectroscopy, J. Magn. Reson., 45, 367–373, https://doi.org/10.1016/0022-2364(81)90137-2, 1981. 
Bodenhausen, G. and Ernst, R. R.: Direct determination of rate constants of slow dynamic processes by two-dimensional “accordion” spectroscopy in nuclear magnetic resonance, J. Am. Chem. Soc., 104, 1304–1309, 1982. 
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Short summary
Multidimensional NMR relaxation experiments provide a powerful means of studying protein dynamics but typically require long acquisition times. Here, we combine two approaches that individually shorten the length of the experiment: accordion spectroscopy and non-uniform sampling (NUS). We extract relaxation rate constants by applying maximum likelihood estimation of sparse exponential modes modeled on the accordion–NUS interferograms, resulting in time savings by a factor of roughly 10.