Articles | Volume 3, issue 1
Magn. Reson., 3, 91–99, 2022
Magn. Reson., 3, 91–99, 2022
Research article
20 May 2022
Research article | 20 May 2022

Imatinib disassembles the regulatory core of Abelson kinase by binding to its ATP site and not by binding to its myristoyl pocket

Stephan Grzesiek et al.

Related subject area

Field: Liquid-state NMR | Topic: Applications – biological macromolecules
Localising nuclear spins by pseudocontact shifts from a single tagging site
Henry W. Orton, Elwy H. Abdelkader, Lydia Topping, Stephen J. Butler, and Gottfried Otting
Magn. Reson., 3, 65–76,,, 2022
Short summary
Localising individual atoms of tryptophan side chains in the metallo-β-lactamase IMP-1 by pseudocontact shifts from paramagnetic lanthanoid tags at multiple sites
Henry W. Orton, Iresha D. Herath, Ansis Maleckis, Shereen Jabar, Monika Szabo, Bim Graham, Colum Breen, Lydia Topping, Stephen J. Butler, and Gottfried Otting
Magn. Reson., 3, 1–13,,, 2022
Short summary
Fluorine NMR study of proline-rich sequences using fluoroprolines
Davy Sinnaeve, Abir Ben Bouzayene, Emile Ottoy, Gert-Jan Hofman, Eva Erdmann, Bruno Linclau, Ilya Kuprov, José C. Martins, Vladimir Torbeev, and Bruno Kieffer
Magn. Reson., 2, 795–813,,, 2021
Short summary
Analysis of conformational exchange processes using methyl-TROSY-based Hahn echo measurements of quadruple-quantum relaxation
Christopher A. Waudby and John Christodoulou
Magn. Reson., 2, 777–793,,, 2021
Short summary
Anomalous amide proton chemical shifts as signatures of hydrogen bonding to aromatic sidechains
Kumaran Baskaran, Colin W. Wilburn, Jonathan R. Wedell, Leonardus M. I. Koharudin, Eldon L. Ulrich, Adam D. Schuyler, Hamid R. Eghbalnia, Angela M. Gronenborn, and Jeffrey C. Hoch
Magn. Reson., 2, 765–775,,, 2021
Short summary

Cited articles

Agafonov, R. V., Wilson, C., Otten, R., Buosi, V., and Kern, D.: Energetic dissection of Gleevec's selectivity toward human tyrosine kinases, Nat. Struct. Mol. Biol., 21, 848–853,, 2014. 
Braun, T. P., Eide, C. A., and Druker, B. J.: Response and Resistance to BCR-ABL1-Targeted Therapies, Cancer Cell, 37, 530–542,, 2020.​​​​​​​ 
Deininger, M. W., Goldman, J. M., and Melo, J. V.: The molecular biology of chronic myeloid leukemia, Blood, 96, 3343–3356, 2000. 
Delaglio, F., Grzesiek, S., Vuister, G. W., Zhu, G., Pfeifer, J., and Bax, A.: NMRPipe: a multidimensional spectral processing system based on UNIX pipes, J. Biomol. NMR, 6, 277–293,, 1995. 
Gasteiger, E., Gattiker, A., Hoogland, C., Ivanyi, I., Appel, R. D., and Bairoch, A.: ExPASy: The proteomics server for in-depth protein knowledge and analysis, Nucleic Acids Res., 31, 3784–3788,, 2003. 
Short summary
We show here that binding of the anticancer drug imatinib to the ATP site of Abelson kinase and not binding to its allosteric site coincides with the opening of the kinase regulatory core at nanomolar concentrations. This has implications for the understanding of Abelson’s kinase regulation and activity during medication as well as for the design of new Abelson kinase inhibitors.