Articles | Volume 4, issue 1
https://doi.org/10.5194/mr-4-115-2023
https://doi.org/10.5194/mr-4-115-2023
Research article
 | 
12 May 2023
Research article |  | 12 May 2023

Time-domain proton-detected local-field NMR for molecular structure determination in complex lipid membranes

Anika Wurl, Kay Saalwächter, and Tiago Mendes Ferreira

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Cited articles

Aisenbrey, C., Salnikov, E., and Bechinger, B.: Solid-State NMR Investigations of the MHC II Transmembrane Domains: Topological Equilibria and Lipid Interactions, J. Mem. Biol., 252, 371–384, https://doi.org/10.1007/s00232-019-00071-8, 2019. a
Andersson, J. M., Grey, C., Larsson, M., Ferreira, T. M., and Sparr, E.: Effect of cholesterol on the molecular structure and transitions in a clinical-grade lung surfactant extract, P. Natl. Acad. Sci. USA, 114, E3592–E3601, https://doi.org/10.1073/pnas.1701239114, 2017. a
Asami, S. and Reif, B.: Comparative Study of REDOR and CPPI Derived Order Parameters by 1H-Detected MAS NMR and MD Simulations, J. Phys. Chem. B, 121, 8719–8730, https://doi.org/10.1021/acs.jpcb.7b06812, 2017. a
Bacle, A., Buslaev, P., Garcia-Fandino, R., Favela-Rosales, F., Ferreira, T., Fuchs, P., Gushchin, I., Javanainen, M., Kiirikki, A., Madsen, J., Melcr, J., Milán Rodríguez, P., Miettinen, M., Ollila, O., Papadopoulos, C., Peón, A., Piggot, T., Piñeiro, A., and Virtanen, S.: Inverse Conformational Selection in Lipid–Protein Binding, J. Am. Chem. Soc., 143, 13701–13709, https://doi.org/10.1021/jacs.1c05549, 2021. a, b, c
Bechinger, B. and Seelig, J.: Conformational changes of the phosphatidylcholine headgroup due to membrane dehydration. A 2H-NMR study, Chemistry and Physics of Lipids, 58, 1–5, https://doi.org/10.1016/0009-3084(91)90105-K, 1991. a, b
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Short summary
R-proton-detected local-field NMR is a powerful method to obtain structural information from biological membrane models. However, the conventional analysis of experiments, by using a Fourier transform in the indirect time-domain and reading-off splittings, is unsuitable to investigate complex systems. One then needs to model the experimental data. Fitting the experimental data with simulations that account for radiofrequency field inhomogeneity enables accurate modeling of R-PDLF data.