Articles | Volume 2, issue 2
© Author(s) 2021. This work is distributed underthe Creative Commons Attribution 4.0 License.
Detecting anisotropic segmental dynamics in disordered proteins by cross-correlated spin relaxation
- Final revised paper (published on 06 Jul 2021)
- Supplement to the final revised paper
- Preprint (discussion started on 08 Apr 2021)
Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor |
: Report abuse
RC1: 'Comment on mr-2021-35', Anonymous Referee #1, 17 Apr 2021
- AC1: 'Reply on RC1', Clemens Kauffmann, 20 Apr 2021
RC2: 'Please see attached file.', Anonymous Referee #2, 22 Apr 2021
- AC2: 'Reply on RC2', Clemens Kauffmann, 27 Apr 2021
RC3: 'Comment on mr-2021-35', Anonymous Referee #2, 01 May 2021
AC3: 'Reply on RC3', Clemens Kauffmann, 05 May 2021
- AC4: 'Reply on AC3', Clemens Kauffmann, 06 May 2021
- AC3: 'Reply on RC3', Clemens Kauffmann, 05 May 2021
Peer review completion
AR: Author's response | RR: Referee report | ED: Editor decision | EF: Editorial file upload
AR by Clemens Kauffmann on behalf of the Authors (25 May 2021) Author's response Author's tracked changes Manuscript
ED: Publish as is (02 Jun 2021) by Daniel Abergel
AR by Clemens Kauffmann on behalf of the Authors (02 Jun 2021) Manuscript
This paper describes the theoretical underpinnings and an experimental approach for obtaining insight into the dynamics of IDPs. Information of this sort is important as the experimental toolkit for IDPs is not as well established as for folded proteins. The authors suggest measuring a pair of cross correlated spin interactions, longitudinal and transverse, from which a factor of Q is calculated. Simulations are provided to establish the information content of Q. The paper is more a description of a conceptual idea – the authors state that pulse sequences for Ca-C’ dipolar, C’ CSA cross-correlation measurements are still in progress and they don’t present experimental data. Therefore, it is difficult for this reviewer to evaluate the utility of the proposed method/idea. For example, in the case of an IDP where little residual structure is present and conformations are in rapid flux, and where diffusion anisotropy could well be present on a per-residue basis, will this method be able to detect this qualitatively? Will a per-residue “feel” (even qualitative) of the dynamics be attainable? Could the authors present some experimental data to try to answer these questions, or the general question of what insights we might be able to obtain practically.
Can the authors comment on whether interactions involving other 13C spins in a U-13C labeled IDP would be predicted to influence the extracted cross-correlation rates, especially in cases where rates are small.