Articles | Volume 2, issue 2
Research article
06 Jul 2021
Research article |  | 06 Jul 2021

Detecting anisotropic segmental dynamics in disordered proteins by cross-correlated spin relaxation

Clemens Kauffmann, Irene Ceccolini, Georg Kontaxis, and Robert Konrat


Interactive discussion

Status: closed

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on mr-2021-35', Anonymous Referee #1, 17 Apr 2021
  • RC2: 'Please see attached file.', Anonymous Referee #2, 22 Apr 2021
  • RC3: 'Comment on mr-2021-35', Anonymous Referee #2, 01 May 2021
    • AC3: 'Reply on RC3', Clemens Kauffmann, 05 May 2021
      • AC4: 'Reply on AC3', Clemens Kauffmann, 06 May 2021

Peer review completion

AR: Author's response | RR: Referee report | ED: Editor decision | EF: Editorial file upload
AR by Clemens Kauffmann on behalf of the Authors (25 May 2021)  Author's response   Author's tracked changes   Manuscript 
ED: Publish as is (02 Jun 2021) by Daniel Abergel
AR by Clemens Kauffmann on behalf of the Authors (02 Jun 2021)  Manuscript 
Short summary
Unlike rigid folded proteins, intrinsically disordered proteins (IDPs) exist as an ensemble of dynamically interconverting structures. Cross-correlated spin-relaxation experiments are particularly suited to characterize the structural dynamics of IDPs as they encode a protein's structural memory. By translating diffusion models of folded proteins to segmental motions in IDPs, we define and assess a sensitive experimental measure for the presence of anisotropic dynamics in the protein backbone.